In-vitro Antioxidant Capacity of Leaves of Tephrosia villosa Linn. (Leguminosae) in Various Solvent Extract

Abstract

Introduction: Leaves of Tephrosia villosa belonging to the family fabaceae being used for the treatment of dropsy and diabetes. The present research work was under taken to investigate the in-vitro antioxidant activity of petroleum ether, chloroform, ethyl acetate, ethanol, methanol, and water extracts. Methods: In vitro antioxidant activities were carried out for six solvent extracts such as T. villosa leaf petroleum ether extract, T. villosa leaf chloroform extract, T. villosa leaf ethylacetate extract, T. villosa leaf ethanol extract, T. villosa leaf methanol extract, and T. villosa leaf water extract by standard methods. Five various antioxidant methods such as DPPH radical scavenging ability, superoxide radical scavenging potential, ABTS scavenging capacity, metal chelating ability, and reducing power assay (FRAP) were estimated. Results: Further investigation was carried out for in-vitro antioxidant activity and radical scavenging assay by calculating its % inhibition by means of IC50 values, all the extracts concentration has been adjusted to come under the linearity range and here reference standards ascorbic acid has been taken for the method suitability. Conclusion: The results revealed that leaves of this plant have antioxidant potential. Among these results, methenolic and water extract has more potent than the other extract. T. villosa Linn. (Fabaceae) leaves possess the antioxidant substance which may be potential responsible for the treatment of oxidative stress related diseases.