The Neuroprotective Effect of Sesame Seed Oil on Cuprizone-Induced Cerebellar Damage in Male Wistar Rats

Authors

  • A. J. Abimbola Department of Anatomy, University of Ilorin, Ilorin, Nigeria. Young Researchers Network, OTABEL Biomedical Solutions, Bauchi, Nigeria
  • A. O. Adebiyi Young Researchers Network, OTABEL Biomedical Solutions, Bauchi, Nigeria, Department of Human Anatomy, Al-Hikmah University, Ilorin, Nigeria.
  • L. O. Oladipupo Department of Anatomy, University of Ilorin, Ilorin, Nigeria. Young Researchers Network, OTABEL Biomedical Solutions, Bauchi, Nigeria
  • F. B. Olawoye Department of Anatomy, University of Ilorin, Ilorin, Nigeria. Young Researchers Network, OTABEL Biomedical Solutions, Bauchi, Nigeria
  • R. M. Kholo Department of Anatomy, University of Ilorin, Ilorin, Nigeria.
  • F. A. Isaac Department of Anatomy, University of Ilorin, Ilorin, Nigeria.
  • I. A. Ayobamidele Department of Anatomy, University of Ilorin, Ilorin, Nigeria.
  • J. O. Bello Young Researchers Network, OTABEL Biomedical Solutions, Bauchi, Nigeria. Department of Human Anatomy, Ahmadu Bello University, Zaria, Nigeria.
  • J. E. Okon Young Researchers Network, OTABEL Biomedical Solutions, Bauchi, Nigeria. Department of Human Anatomy, University of Uyo, Uyo, Nigeria.
  • A. M. Ahmad Young Researchers Network, OTABEL Biomedical Solutions, Bauchi, Nigeria. Department of Human Physiology, Sa’adu Zugur University, Gadau, Nigeria.
  • G. O. Omotoso Department of Anatomy, University of Ilorin, Ilorin, Nigeria.

DOI:

https://doi.org/10.21276/apjhs.2025.12.4.06

Keywords:

Cerebellar damage, Cuprizone, Demyelination, Oxidative enzymes, Sesame seed oil

Abstract

Cuprizone (CZ) is a copper chelator often used in laboratory research to induce demyelination in animal models. It helps to study the processes involved in demyelination and remyelination, which are relevant to multiple sclerosis research and other demyelinating diseases. This study explored the effects of Sesame seed oil (SSO) on oxidative, histomorphological and behavioral changes in CZ-damaged cerebellum. Twenty-four adult male Wistar rats were purchased and acclimatized for a week. They were, however, grouped into four and administered the following, respectively; Group A received standard rat feed (control), Group B received 2 g of CZ per 1 kg of rat/day, Group C received 5 mL SSO per 1 kg of rat/day, and Group D received 2 g of CZ per 1 kg of rat/day + 5 mL SSO per 1 kg of rat/day. This was done consecutively for 3 weeks with the use of oral cannula. The animals were assessed for exploratory and locomotor activities while the cerebellum was processed for histology, assayed for catalase chloramphenicol acetyltransferase (CAT) and superoxide dismutase (SOD) activities with immunohistochemical using ionized calcium-binding adaptor molecule 1 (IBA1). CZ treatment caused weight reduction, disruption of Purkinje cell layer, cellular degeneration, and reduction in CAT and SOD activities with IBA staining. However, these changes were ameliorated when co-administered with SSO. The results suggested that SSO contains potent anti-oxidant and anti-inflammatory characteristics that can help to alleviate the harmful effects of CZ in various regions of the body.

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Published

2025-09-30

How to Cite

A. J. Abimbola, A. O. Adebiyi, L. O. Oladipupo, F. B. Olawoye, R. M. Kholo, F. A. Isaac, I. A. Ayobamidele, J. O. Bello, J. E. Okon, A. M. Ahmad, & G. O. Omotoso. (2025). The Neuroprotective Effect of Sesame Seed Oil on Cuprizone-Induced Cerebellar Damage in Male Wistar Rats. Asian Pacific Journal of Health Sciences, 12(4), 39–44. https://doi.org/10.21276/apjhs.2025.12.4.06

Issue

Vol. 12 No. 4 (2025): Vol 12 | Issue 4 | October - December 2025

Section

Articles

License

Copyright (c) 2025 A. J. Abimbola, A. O. Adebiyi, L. O. Oladipupo, F. B. Olawoye, R. M. Kholo, F. A. Isaac, I. A. Ayobamidele, J. O. Bello, J. E. Okon, A. M. Ahmad, G. O. Omotoso

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Asian Pacific Journal of Health Sciences applies the Creative Commons Attribution (CC-BY) license to published articles. Under this license, authors retain ownership of the copyright for their content, but they allow anyone to download, reuse, reprint, modify, distribute and/or copy the content as long as the original authors and source are cited. Appropriate attribution can be provided by simply citing the original article.